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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIA Mouse 15K cDNA Clone Set
Organism:Mus musculus
Strain:C57BL/6J
UniGene Library ID:8622
Tissue Description:
Library Keywords:ovary, normal, bulk, unfertilized ovum, mesonephros, neonate, blastocyst, EST, mixed tissue, embryonic tissue, mixed developmental stages, extraembryonic tissue, plasmid vector, oligo-dT primed, C57BL/6J

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:26636

Library Preparation Details

Description:This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978.
R. Site1:SalI
R. Site2:NotI
Lab Host:DH10B
Vector:pSPORT1
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: