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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:neural progenitor enriched library
Organism:Mus musculus
Strain:CD1
UniGene Library ID:7264
Tissue Description:
Library Keywords:normal, cerebral cortex, cell line, subtracted, EST, neurosphere, unknown developmental stage, bFGF, plasmid vector, CD-1

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:340

Library Preparation Details

Description:Cells were harvested from P0 mouse cortex and cultured for 4 weeks in the presence of bFGF to grow neurospheres. Half of the cells were then placed under differentiating conditions for 24 hours. RNA was extracted from both groups using acid phenol extraction, PolyA(+) RNA was purified using oligo dt paramagnetic beads, and cDNA was synthesized. Amplicons were created from Dpn II digested cDNA and 2 rounds of RDA subtraction were performed using amplicons from the differentiated cells as the driver. Resultant library, enriched in clones from the undifferentiated neural progenitors, was then shotgun cloned into BamH1 digested Bluescript SK II+ and transformed into E. coli.
R. Site1:BamHI
R. Site2:
Lab Host:Hbl electromax DH10B (GibcoBrl)
Vector:pBluescript KS II+
Vector Type:Plasmid
Tissue Supplier:
Library Producer: