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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:RIKEN full-length enriched, adult male lung
Organism:Mus musculus
Strain:
UniGene Library ID:7216
Tissue Description:
Library Keywords:lung, normal, bulk, adult, full-length enriched, EST, male

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:5983

Library Preparation Details

Description:cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGAAGGATCCAAGAGCTCAATTAATTAATTAAACCCCCCCCCCC 3']. cDNA was cleaved with XhoI and SstI.
R. Site1:XhoI
R. Site2:SstI
Lab Host:SOLR
Vector:
Vector Type:
Tissue Supplier:
Library Producer: