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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:McCarrey Eddy spermatocytes
Organism:Mus musculus
UniGene Library ID:6787
Tissue Description:
Library Keywords:testis, normal, bulk, adult, spermatocyte, EST, male, size fractionated, directionally cloned, phagemid, oligo-dT primed, CD-1

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:4385

Library Preparation Details

Description:cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of >400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library contains 98% recombinants. References: J. Androl. 20:635-639 and Gene 25:263-269. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences). Original lambda-based library is available through ATCC, catalog #63422.
R. Site1:XhoI
R. Site2:EcoRI
Lab Host:DH10B (phage-resistant)
Vector:pBluescript SK+ (Stratagene)
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: