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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Soares_NKWMD_mandible
Organism:Mus musculus
Strain:
UniGene Library ID:6536
Tissue Description:
Library Keywords:normal, bulk, normalized, EST, Soares normalized, mandible, unknown developmental stage, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:3020

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCTTAATTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.
R. Site1:NotI
R. Site2:EcoRI
Lab Host:DH10B (phage-resistant)
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: