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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Sp2
Organism:Mus musculus
Strain:
UniGene Library ID:7324
Tissue Description:Spleen, mouse spleen NK cells (NK01)
Library Keywords:spleen, normal, non-normalized, flow-sorted, natural killer cell, CGAP, EST, LTI non-normalized, unknown developmental stage, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:768
#Sequences Generated to Date:949

Library Preparation Details

Description:mRNA made from flow-sorted NK cells, cDNA made by oligo-dT priming. Directionally cloned. Average insert size 1.5 kb. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D.
R. Site1:
R. Site2:
Lab Host:DH10B (T1-resistant)
Vector:pCMV-SPORT6 (Life Technologies)
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:David Segal Ph.D., Herbert Morse M.D.
Library Producer:Life Technologies, Inc.