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Library Info Page


Library ID

Library Name:RIKEN full-length enriched, adult pancreas Islet cells
Organism:Mus musculus
Strain:
UniGene Library ID:5429
Tissue Description:
Library Keywords:pancreatic islet, normal, bulk, adult, full-length enriched, EST

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:3562

Library Preparation Details

Description:cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.~Islet cells were provided by Hiroo Iwata, Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto, 606-8507 Japan, whose assistance we gratefully acknowledge.
R. Site1:SalI
R. Site2:BamHI
Lab Host:DH10B
Vector:
Vector Type:
Tissue Supplier:
Library Producer: