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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:RIKEN full-length enriched, 6 days neonate skin
Organism:Mus musculus
UniGene Library ID:5358
Tissue Description:
Library Keywords:skin, normal, bulk, normalized, subtracted, juvenile, full-length enriched, EST

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:2434

Library Preparation Details

Description:cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 185.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.
R. Site1:SalI
R. Site2:BamHI
Lab Host:DH10B
Vector Type:
Tissue Supplier:
Library Producer: