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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Soares_mouse_NMIE
Organism:Mus musculus
Strain:C3H x 101 F1
UniGene Library ID:4088
Tissue Description:
Library Keywords:normal, bulk, normalized, neonate, EST, Soares normalized, male, inner ear, phagemid, oligo-dT primed, crossbred

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:1873

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCACACTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed and donated by Bento Soares and M.Fatima Bonaldo (University of Iowa) and R. Hardisty, A. Varela-Carver, P. Mburu and S.D.M. Brown (MRC UK Mouse Genome Centre and Mammalian Genetics Unit, Harwell, UK).
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: