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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Ren Stubbs mouse thymus
Organism:Mus musculus
Strain:C3H
UniGene Library ID:4086
Tissue Description:
Library Keywords:thymus, normal, bulk, juvenile, EST, mixed pool by gender, size fractionated, phagemid, oligo-dT primed, C3H

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:1189

Library Preparation Details

Description:1st strand cDNA was primed with an oligo(dT) primer; double-stranded cDNA was ligated using 5' linker ggccgctat and 3' linker aactggaagcttaatt. Library is size-selected >2.5 kb and average insert size is 3.5 kb. Clones were arrayed from primary plating; non-amplified. Library constructed by X. Ren and L. Stubbs (Lawrence Livermore National Laboratory and DOE Joint Genome Institute, 7000 East Ave, L-453, Livermore, CA 94550).
R. Site1:NotI
R. Site2:PacI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: