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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Schiller mouse MAC13
Organism:Mus musculus
Strain:
UniGene Library ID:1409
Tissue Description:
Library Keywords:colon, cell line, EST, unknown developmental stage, size fractionated, plasmid vector, colorectal cancer

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:651

Library Preparation Details

Description:Double-stranded cDNA was prepared from cell line MAC13 using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:SOLR
Vector:pBluescript SK- (Stratagene)
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: