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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:Soares_NMGBC_B-cell
Organism:Mus musculus
UniGene Library ID:4263
Tissue Description:Lymph, germinal B-cell from resting spleen
Library Keywords:spleen, B-cell, normal, flow-sorted, normalized, CGAP, EST, Soares normalized, unknown developmental stage, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:1536
#Sequences Generated to Date:2320

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGGATTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo.
R. Site1:NotI
R. Site2:EcoRI
Lab Host:DH10B (phage resistant)
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:M. Bento Soares, Ph.D.
Library Producer:Soares Laboratory