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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Soares_embryonic_stem_cell_NMES
Organism:Mus musculus
Strain:
UniGene Library ID:1330
Tissue Description:Embryonic stem cell
Library Keywords:normal, cell line, normalized, CGAP, EST, Soares normalized, embryonic stem cell, embryonic tissue, unknown embryonic stage, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:1152
#Sequences Generated to Date:924

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through two rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Marcello Bento Soares, Ph.D.
Library Producer:Soares Laboratory