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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Soares_NMPu
Organism:Mus musculus
Strain:
UniGene Library ID:1097
Tissue Description:Uterus, pregnant mouse
Library Keywords:uterus, normal, bulk, pregnant, adult, normalized, CGAP, EST, Soares normalized, MGC, female, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:8064
#Sequences Generated to Date:6589

Library Preparation Details

Description:1st strand cDNA was prepared from pregnant mouse uterus, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo.
R. Site1:
R. Site2:
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Marcelo Bento Soares, Ph.D.
Library Producer:Soares Laboratory