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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Knowles Solter mouse blastocyst B3
Organism:Mus musculus
Strain:C57BL/6J x DBA/2J F1
UniGene Library ID:875
Tissue Description:
Library Keywords:normal, micro-bulk, blastocyst, EST, embryonic tissue, size fractionated, directionally cloned, phagemid, oligo-dT primed, crossbred

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:2499

Library Preparation Details

Description:Cloned unidirectionally from mRNA prepared from 800 blastocysts. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the NotI/SalI sites of a pSPORT vector (Life Technologies). Two different size selections: B1 (larger inserts) and B3.
R. Site1:NotI
R. Site2:SalI
Lab Host:DH10B
Vector:pSPORT
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: