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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Knowles Solter mouse 2 cell
Organism:Mus musculus
Strain:C57BL/6J x DBA/2J F1
UniGene Library ID:862
Tissue Description:
Library Keywords:normal, micro-bulk, EST, embryonic tissue, early cleavage, size fractionated, directionally cloned, oligo-dT primed, crossbred

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:15277

Library Preparation Details

Description:Cloned unidirectionally from mRNA prepared from 13,500 2-cell stage embryos. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the MluI/SalI sites of a modified pBluescribe vector using commercial linkers (NEB). Average insert size: 1.2 kb.
R. Site1:MluI
R. Site2:SalI
Lab Host:DH10B
Vector:pBluescribe (modified)
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: