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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NIH_MGC_17
Organism:Homo sapiens
UniGene Library ID:3714
Tissue Description:Muscle, rhabdomyosarcoma
Library Keywords:muscle, rhabdomyosarcoma, non-normalized, cell line, EST, unknown developmental stage, MGC, Rubin non-normalized, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:31872
#Sequences Generated to Date:26443

Library Preparation Details

Description:cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies).
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:DH10B (phage-resistant)
Vector Type:plasmid (chloramphenicol resistant)
Tissue Supplier:
Library Producer:Rubin Laboratory