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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_GCB0
Organism:Homo sapiens
UniGene Library ID:1051
Tissue Description:Lymph, normal germinal center B cells
Library Keywords:B-cell, normal, non-normalized, flow-sorted, CGAP, EST, tonsil, unknown developmental stage, Staudt non-normalized

Clones and Sequences

#Clones Generated to Date:3456
#Sequences Generated to Date:907

Library Preparation Details

Description:Cloned unidirectionally. Primer: Oligo dT. Germinal center B-cells Library constructed by Dr. L. Staudt (NCI). 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.1 kb.
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:SOLR (kanamycin resistant)
Vector:Bluescript SK-
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Louis M. Staudt, M.D., Ph.D., David Allman, Ph.D., Gerald Marti, M.D.
Library Producer:Staudt Laboratory