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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NCI_CGAP_CLL1
Organism:Homo sapiens
UniGene Library ID:1317
Tissue Description:Blood, chronic lymphocytic leukemia cells. PolyA+ mRNA prepared from the peripheral blood of five patients using magnetic cell sorting for CD19+ cells.
Library Keywords:B-cell, chronic lymphocytic leukemia, uncharacterized tissue, flow-sorted, normalized, CGAP, EST, Soares normalized, unknown developmental stage, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:22272
#Sequences Generated to Date:13690

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTGCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed by Bento Soares and M.Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Ash Alizadeh, John Byrd, M.D., Mike Grever, M.D., Louis M. Staudt, M.D., Ph.D.
Library Producer:Soares Laboratory