Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:KG1-a Lambda Zap Express cDNA library
Organism:Homo sapiens
UniGene Library ID:599
Tissue Description:
Library Keywords:bone marrow, non-normalized, cell line, adult, acute myelogenous leukemia, EST, male, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:880

Library Preparation Details

Description:Unidirectional cloning sites: EcoR1-Xho1. mRNA was purified from KG1-a cell line, cDNA was synthesized using an Xho1-OligodT linker primer. EcoR1 adaptors were ligated, followed by digestion with Xho1 for directional cloning into predigested Lambda Zap Express
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:
Vector:Lambda Zap Express (Stratagene)
Vector Type:
Tissue Supplier:
Library Producer: