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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NCI_CGAP_Pr25
Organism:Homo sapiens
UniGene Library ID:1017
Tissue Description:Prostate, normal epithelium, HPV transformed
Library Keywords:prostate, epithelium, normal, non-normalized, cell line, CGAP, EST, Stratagene non-normalized, unknown developmental stage, male, size fractionated, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:3456
#Sequences Generated to Date:1883

Library Preparation Details

Description:Cloned unidirectionally. Primer: Oligo dT. Normal prostate epithelial cell line (HPV immortalized). 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.1 kb.
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:SOLR (kanamycin resistant)
Vector:Bluescript SK-
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Suzanne L. Topalian, M.D., Robert K. Bright, Ph.D.
Library Producer:Stratagene, Inc.