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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Lym3
Organism:Homo sapiens
UniGene Library ID:1015
Tissue Description:Lymph, lymphoma, 10 pooled samples, including broad spectrum of lymphoma tumor types
Library Keywords:lymph node, lymphoma, non-normalized, bulk, CGAP, EST, Stratagene non-normalized, unknown developmental stage, size fractionated, directionally cloned, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:1152
#Sequences Generated to Date:654

Library Preparation Details

Description:Cloned unidirectionally. Primer: Oligo dT. Pooled lymphomas. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 0.9 kb.
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:SOLR (kanamycin resistant)
Vector:Bluescript SK-
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Mark Raffeld, M.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Stratagene, Inc.