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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:Stratagene HeLa cell s3 937216
Organism:Homo sapiens
UniGene Library ID:527
Tissue Description:
Library Keywords:cervix, epithelium, non-normalized, cell line, adult, EST, female, phagemid, oligo-dT primed, cervical adenocarcinoma

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:7073

Library Preparation Details

Description:Cloned unidirectionally. Primer: Oligo dT. HeLa S3 epithelioid carcinoma cells grown to semi-confluency without induction. Average insert size: 1.5 kb; Uni-ZAP XR Vector. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:SOLR (kanamycin resistant)
Vector:pBluescript SK-
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: