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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:Stratagene neuroepithelium NT2RAMI 937234
Organism:Homo sapiens
UniGene Library ID:524
Tissue Description:
Library Keywords:metastasis, testis, non-normalized, cell line, adult, retinoic acid, embryonal carcinoma, EST, male, size fractionated, directionally cloned, phagemid, oligo-dT primed, neuroepithelium

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:2329

Library Preparation Details

Description:Cloned unidirectionally. Primer: Oligo dT. NT2 (Ntera-2/c1.D1) precursor cells induced with Retinoic Acid for 1 week, followed by 3 weeks in mitotic inhibitors (Replate #2). Average insert size: 1.1 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:SOLR (kanamycin resistant)
Vector:pBluescript SK-
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: