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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_HSC1
Organism:Homo sapiens
UniGene Library ID:933
Tissue Description:Blood, stem cells. CD34+/CD38- fraction from flow sorted human bone marrow
Library Keywords:bone marrow, normal, non-normalized, flow-sorted, hematopoietic cell, CGAP, EST, Krizman protocol 1, unknown developmental stage, size fractionated, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:2304
#Sequences Generated to Date:824

Library Preparation Details

Description:mRNA made from flow-sorted CD34+/CD38- hematopoietic stem cells, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center
R. Site1:
R. Site2:
Lab Host:DH10B
Vector:pAMP10
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:Donald Orlic, Ph.D.
Library Producer:Krizman Laboratory