Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
Tissues
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Co12
Organism:Homo sapiens
UniGene Library ID:988
Tissue Description:Colon, 10 pooled tumors, including broad spectrum of colon tumor types
Library Keywords:non-normalized, colon, bulk, CGAP, EST, Stratagene non-normalized, unknown developmental stage, mixed pool by gender, directionally cloned, phagemid, oligo-dT primed, colorectal cancer

Clones and Sequences

#Clones Generated to Date:8448
#Sequences Generated to Date:2801

Library Preparation Details

Description:Cloned unidirectionally. Primer: Oligo dT. Pooled colon tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.2 kb.
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:SOLR (kanamycin resistant)
Vector:Bluescript SK-
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:L. Jeffrey Medeiros, M.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Stratagene, Inc.