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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_GC5
Organism:Homo sapiens
UniGene Library ID:990
Tissue Description:Germ cell tumor, pooled from mixed types including seminoma, embryonal cell, and teratoma with adenocarcinoma
Library Keywords:germ cell tumor, non-normalized, uncharacterized tissue, bulk, CGAP, EST, Stratagene non-normalized, unknown developmental stage, directionally cloned, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:1536
#Sequences Generated to Date:1191

Library Preparation Details

Description:Cloned unidirectionally. Primer: Oligo dT. Mixed germ cell tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 0.7 kb.
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:SOLR (kanamycin resistant)
Vector:Bluescript SK-
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Stratagene, Inc.