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Cancer Genome Characterization Initiative

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Library Info Page

Library ID

Library Name:Morton Fetal Cochlea
Organism:Homo sapiens
UniGene Library ID:371
Tissue Description:
Library Keywords:fetus, normal, non-normalized, cochlea, bulk, EST, size fractionated, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:14805

Library Preparation Details

Description:Reference: Genomics 23, 42-50 (1994) Cloned unidirectionally. Primer: Oligo dT. Fetal cochlea, normal. 37% of inserts <0.5 kb, 56% 0.5-1.0 kb, 7% >1 kb. Uni-ZAP XR Vector. Library constructed by N. Robertson, C. Morton. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:SOLR cells (kanamycin resistant)
Vector:pBluescript SK-
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: