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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Br3
Organism:Homo sapiens
UniGene Library ID:986
Tissue Description:Breast, ductal, poorly differentiated
Library Keywords:mammary gland, invasive, poorly differentiated, non-normalized, bulk, CGAP, EST, Stratagene non-normalized, unknown developmental stage, female, directionally cloned, phagemid, oligo-dT primed, ductal carcinoma of the breast

Clones and Sequences

#Clones Generated to Date:1920
#Sequences Generated to Date:1017

Library Preparation Details

Description:Cloned unidirectionally. Primer: Oligo dT. Ductal breast tumor. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 0.9 kb.
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:SOLR (kanamycin resistant)
Vector:Bluescript SK-
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:L. Jeffrey Medeiros, M.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Stratagene, Inc.