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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Soares retina N2b4HR
Organism:Homo sapiens
UniGene Library ID:302
Tissue Description:
Library Keywords:retina, normal, non-normalized, bulk, adult, EST, male, size fractionated, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:12538

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). The retinas were obtained from a 55 year old Caucasian and total cellular poly(A)+ RNA was extracted 6 hrs after their removal. The retina RNA was kindly provided by Roderick R. McInnes M.D. Ph.D. from the University of Toronto. Library constructed by Bento Soares and M.Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B (ampicillin resistant)
Vector:pT7T3D (Pharmacia) with a modified polylinker
Vector Type:phagemid
Tissue Supplier:
Library Producer: