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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Soares breast 3NbHBst
Organism:Homo sapiens
UniGene Library ID:298
Tissue Description:
Library Keywords:mammary gland, normal, bulk, adult, normalized, EST, Soares normalized, female, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:9870

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 20. Library constructed by Bento Soares and M.Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B (ampicillin resistant)
Vector:pT7T3D (Pharmacia) with a modified polylinker
Vector Type:phagemid
Tissue Supplier:
Library Producer: