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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_GCB1
Organism:Homo sapiens
UniGene Library ID:595
Tissue Description:Lymph, normal germinal center B cells
Library Keywords:lymph node, B-cell, normal, flow-sorted, normalized, CGAP, EST, Soares normalized, unknown developmental stage, MGC

Clones and Sequences

#Clones Generated to Date:73340
#Sequences Generated to Date:52246

Library Preparation Details

Description:1st strand cDNA was prepared from human tonsillar cells enriched for germinal center B cells by flow sorting (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr. David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA synthesis was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT-3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Louis M. Staudt, M.D., Ph.D., David Allman, Ph.D., Gerald Marti, M.D.
Library Producer:Soares Laboratory