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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Fetal heart
Organism:Homo sapiens
UniGene Library ID:276
Tissue Description:
Library Keywords:heart, normal, non-normalized, bulk, EST, first trimester, phage vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:1935

Library Preparation Details

Description:mRNA was purified from human fetal hearts (10-12 weeks). cDNA was constructed using a NotI-Oligo dT adaptor-primer. SalI adaptors were ligated, followed by digestion with NotI, for direction cloning into predigested lambda gt22. Method is described in J. Mol. Cell. Cardiol. (1994) 26, 1329-1333)
R. Site1:NotI
R. Site2:SalI
Lab Host:E. coli Y1090
Vector:Lambda gt22
Vector Type:Phage
Tissue Supplier:
Library Producer: