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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Human retina cDNA Tsp509I-cleaved sublibrary
Organism:Homo sapiens
UniGene Library ID:432
Tissue Description:
Library Keywords:retina, normal, non-normalized, bulk, adult, EST, mixed pool by gender, size fractionated

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:1524

Library Preparation Details

Description:The library used for sequencing was a sublibrary derived from a human retina cDNA library. Inserts from retina cDNA library DNA were isolated, cleaved with Tsp509I, size selected, and cloned into lamda gt10. Individual plaques were arrayed and used as templates for PCR amplification and these PCR products were used for sequencing.
R. Site1:EcoRI
R. Site2:EcoRI
Lab Host:E. coli strain K802
Vector:lambda gt10
Vector Type:
Tissue Supplier:
Library Producer: