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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Co9
Organism:Homo sapiens
UniGene Library ID:987
Tissue Description:Colon cancer
Library Keywords:non-normalized, colon, bulk, CGAP, EST, Soares non-normalized, unknown developmental stage, phagemid, colorectal cancer

Clones and Sequences

#Clones Generated to Date:6144
#Sequences Generated to Date:3802

Library Preparation Details

Description:1st strand cDNA was prepared from RER+ colon tumor, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is not normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo (Soares4).
R. Site1:
R. Site2:
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier: Ilan Kirsch, M.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Soares Laboratory