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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:Soares infant brain 1NIB
Organism:Homo sapiens
UniGene Library ID:263
Tissue Description:
Library Keywords:normal, bulk, normalized, EST, Soares normalized, female, infant, directionally cloned, oligo-dT primed, whole brain

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:47763

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Hind III adaptors (Pharmacia), digested with Not I and directionally cloned into the Not I and Hind III sites of the Lafmid BA vector. Library went through one round of normalization. Library constructed by Bento Soares and M.Fatima Bonaldo.
R. Site1:Not I
R. Site2:Hind III
Lab Host:DH10B (ampicillin resistant)
Vector:Lafmid BA
Vector Type:phagemid
Tissue Supplier:
Library Producer: