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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Lei2
Organism:Homo sapiens
UniGene Library ID:1187
Tissue Description:Muscle, 2 pooled leiomyosarcomas
Library Keywords:smooth muscle, leiomyosarcoma, uncharacterized tissue, bulk, normalized, CGAP, EST, Soares normalized, unknown developmental stage, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:16896
#Sequences Generated to Date:3320

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-AACTGGAAGAATTCGCGGCCGCAATCGTTTTTTTTTTTTTTTTTTT-3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Christopher A. Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Soares Laboratory