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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Human gastric cancer subtracted library
Organism:Homo sapiens
UniGene Library ID:21976
Tissue Description:
Library Keywords:stomach, adult, subtracted, EST, female, plasmid vector, gastric adenocarcinoma

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:340

Library Preparation Details

Description:Library was prepared using Clontech PCR-Select cDNA Subtraction Kit (BD Bioscience) starting from total RNA isolated from tumor and adjacent non-tumor gastric tissue. We constructed subtracted cDNA library by hybridizing non-tumor (driver) and tumor (tester) cDNAs, obtaining up-regulated sequences in cancerous tissue. Subtracted library of up-regulated genes was cloned into pCRII plasmids using TA cloning kit (Invitrogen). Sequence information was obtained for all clones.
R. Site1:
R. Site2:
Lab Host:One Shot Competent Cells
Vector:pCRII
Vector Type:Plasmid
Tissue Supplier:
Library Producer: