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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Outward Alu-primed hncDNA library
Organism:Homo sapiens
UniGene Library ID:247
Tissue Description:
Library Keywords:uncharacterized tissue, uncharacterized preparation, uncharacterized histology, EST, unknown developmental stage

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:382

Library Preparation Details

Description:The library was constructed as described in [Obradovic, D., Borodin, A.M., Kopantzev, E.P., Wagner, L.L., Volik, S.V., Ermolaeva, O.D., Lebedev, Y.B., Monastyrskaya, G.S., Sverdlov, E.D. (1993) Bioorganicheskaya khimia, 20, 919-930]. This protocol is based on nested primer strategy using Alu- specific primers (ALN3 and TC-65) that direct the hncDNA synthesis outward of Alu repeats.
R. Site1:EcoR1
R. Site2:BamH1
Lab Host:
Vector:pGEM-3Z
Vector Type:Plasmid
Tissue Supplier:
Library Producer: