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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_GC4
Organism:Homo sapiens
UniGene Library ID:1050
Tissue Description:Germ cell tumors, 3 pooled samples, including broad spectrum germ cell tumor types
Library Keywords:germ cell tumor, uncharacterized tissue, bulk, normalized, CGAP, EST, Soares normalized, unknown developmental stage, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:16896
#Sequences Generated to Date:10166

Library Preparation Details

Description:1st strand cDNA was prepared from 3 pooled germ cell tumors, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo.
R. Site1:
R. Site2:
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Christopher A. Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Soares Laboratory