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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:HT29M6
Organism:Homo sapiens
UniGene Library ID:197
Tissue Description:
Library Keywords:colon, cell line, EST, directionally cloned, oligo-dT primed, adenocarcinoma of the colon

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:32

Library Preparation Details

Description:Poly A+ RNA was extracted from the colon adenocarcinoma cell line HT29M6. cDNA synthesis was primed with oligo-dT and the products were directionally cloned in the lambda-ZAPII vector.
R. Site1:Eco RI
R. Site2:Xho I
Lab Host:E. coli XL1Blue
Vector:Lambda ZAPII
Vector Type:Phage
Tissue Supplier:
Library Producer: