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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:Testis 1
Organism:Homo sapiens
UniGene Library ID:119
Tissue Description:
Library Keywords:testis, normal, bulk, adult, normalized, EST, male, plasmid vector, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:433

Library Preparation Details

Description:mRNA was prepared from human testis of a 27 years old man. cDNA was prepared using a 15mer oligo dT anchored by two degenerated bases at its 3'end and containing a NotI site at its 5'end. The cDNA was cloned between EcoRV and NotI sites of PGEM 5zf(+). The 3' end is at the NotI site. The EcoRV site is lost during the cloning procedure. cDNA corresponding to abundant species were eliminated from this library.
R. Site1:EcoRV
R. Site2:Not1
Lab Host:
Vector:PGEM 5zf(+)
Vector Type:Plasmid
Tissue Supplier:
Library Producer: