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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Human cultured dermal papilla cell cDNA library(B)
Organism:Homo sapiens
UniGene Library ID:21096
Tissue Description:
Library Keywords:skin, normal, EST, size fractionated, plasmid vector, directionally cloned, oligo-dT primed, dermal papilla cell

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:1412

Library Preparation Details

Description:Library was constructed using ZAP-cDNA synthesis kit (Stratagene). mRNA was prepared from cultured dermal papilla cells which isolated from beard. Cloned unidirectionally. Primer: Oligo dT. Average insert size: 1.5 kb. Uni-ZAP XR vector: 5' adaptor sequence, 5'-GAATTCGGCACGAG-3'; 3' adaptor sequence, 5'-CTCGAGTTTTTTTTTTTTTTTTTT-3'. Size fractionated to remove sequence <500 bp in size. Amplified once.
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:SOLR
Vector:pBluescript SK-
Vector Type:plasmid
Tissue Supplier:
Library Producer: