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Cancer Genome Characterization Initiative

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Library Info Page

Library ID

Library Name:Human optic nerve. Unnormalized (nbj)
Organism:Homo sapiens
UniGene Library ID:19471
Tissue Description:
Library Keywords:normal, adult, optic nerve, EST, plasmid vector, directionally cloned

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:3652

Library Preparation Details

Description:RNA was extracted from pooled human optic nerve. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual ( First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through
R. Site1:
R. Site2:
Lab Host:EMDH10B
Vector Type:Plasmid
Tissue Supplier:
Library Producer: