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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_281
Organism:Homo sapiens
UniGene Library ID:16677
Tissue Description:Embryonic stem cells, cell line (HSF-6, NIH Registry designation UC06) derived from blastocyst stage embryo cells. Inner cell mass of blastocyst.
Library Keywords:normal, cell line, normalized, blastocyst, EST, embryonic stem cell, MGC, embryonic tissue, size fractionated, plasmid vector, HSF6

Clones and Sequences

#Clones Generated to Date:9984
#Sequences Generated to Date:4371

Library Preparation Details

Description:RNA obtained from pluripotent cell line derived from blastocyst inner cell mass (cell line HSF-6, NIH Registry designation UC06. Positive for OCT4 expression by rtPCR, positive for SSEA-3, SSEA-4, Tra-1-81, Tra-1-60 by immunofluorescence. Negative for SSEA-1 by immunofluorescence Passage 62. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25 kb resulted in an average insert size of 2.0 kb. This primary library is normalized (non-normalized primary library is NIH_MGC_280) and was constructed by Express Genomics (Frederick, MD). Note: this is a Mammalian Gene Collection library.
R. Site1:EcoRV
R. Site2:NotI
Lab Host:DH10B TonA
Vector:pExpress-1
Vector Type:plasmid
Tissue Supplier:
Library Producer:Express Genomics