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Library Info Page


Library ID

Library Name:Human keratoconus cornea, unamplified, (od/oe)
Organism:Homo sapiens
UniGene Library ID:16572
Tissue Description:
Library Keywords:cornea, bulk, adult, EST, plasmid vector, keratoconus

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:6866

Library Preparation Details

Description:Approximately 40ug total RNA was extracted from 7 adult human keratoconus corneas. A directionally cloned cDNA library in the pSPORT1 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).
R. Site1:
R. Site2:
Lab Host:EMDH10B
Vector:pCMVSport6
Vector Type:Plasmid
Tissue Supplier:
Library Producer: