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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_262
Organism:Homo sapiens
UniGene Library ID:16981
Tissue Description:Embryonic stem cells, isolated from the Inner Cell Mass of Blastocyst stage embryos and differentiated to an early neural progentior cell type, BG01, Nestin+, Musashi+-, Passage number:- 18
Library Keywords:normal, non-normalized, cell line, EST, embryonic stem cell, MGC, embryonic tissue, male, unknown embryonic stage, plasmid vector, hESBGN-01

Clones and Sequences

#Clones Generated to Date:14976
#Sequences Generated to Date:12858

Library Preparation Details

Description:RNA obtained from human embryonic stem cells isolated from the inner cell mass of blastocyst stage embryos and differentiated to an early neural progenitor cell type. Cell line id and NIH Registry designation is BGO1. Positive for Nestin and Musashi expression. Passage number 18. cDNA primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. This primary library is non-normalized (normalized primary library is NIH_MGC_259). It was constructed by Express Genomics (Frederick, MD). Sequence ends have been trimmed to exclude vector and regions below Phred quality 16. Note: this is a Mammalian Gene Collection library.
R. Site1:NotI
R. Site2:EcoRV
Lab Host:DH10B-T1 phage-resistant E. coli
Vector:pExpress-1
Vector Type:Plasmid
Tissue Supplier:
Library Producer:Express Genomics