Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
Tissues
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Br2
Organism:Homo sapiens
UniGene Library ID:895
Tissue Description:Breast, 3 pooled tumors, including broad spectrum of breast tumor types
Library Keywords:mammary gland, bulk, normalized, CGAP, EST, Soares normalized, unknown developmental stage, female, phagemid, oligo-dT primed, breast (mammary gland) cancer

Clones and Sequences

#Clones Generated to Date:13440
#Sequences Generated to Date:7519

Library Preparation Details

Description:1st strand cDNA was prepared from pooled bulk breast tumor tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. This library is the normalized version of NCI_CGAP_Br1.1. Library was constructed by Bento Soares and M. Fatima Bonaldo.
R. Site1:
R. Site2:
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Soares Laboratory