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Library Info Page


Library ID

Library Name:NIH_MGC_228
Organism:Homo sapiens
UniGene Library ID:14591
Tissue Description:Brain, cerebellar cortex
Library Keywords:normal, bulk, full-length enriched, EST, unknown developmental stage, MGC, size fractionated, plasmid vector, oligo-dT primed, cerebellar cortex

Clones and Sequences

#Clones Generated to Date:2304
#Sequences Generated to Date:1653

Library Preparation Details

Description:Library is oligo-dT primed and directionally cloned.5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'5'-ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)3N-1N-3. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected for >0.5kb with an average insert size of 1.2kb Library created in the laboratory of Jonathan Kuo and Ted Usdin.
R. Site1:SfiI (directional)
R. Site2:SfiI (directional)
Lab Host:DH10B TonA
Vector:pDNR-LIB
Vector Type:plasmid
Tissue Supplier:
Library Producer:Michael Brownstein / Ted Usdin Laboratory