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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_227
Organism:Homo sapiens
UniGene Library ID:14590
Tissue Description:Spinal cord
Library Keywords:spinal cord, normal, bulk, full-length enriched, EST, unknown developmental stage, MGC, size fractionated, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:2304
#Sequences Generated to Date:1566

Library Preparation Details

Description:Library is oligo-dT primed and directionally cloned.5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30N-1N-3. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected for >0.5kb with an average insert size of 1.3kb Library created in the laboratory of Jonathan Kuo and Ted Usdin.
R. Site1:SfiI (directional)
R. Site2:SfiI (directional)
Lab Host:DH10B TonA
Vector:pDNR-LIB
Vector Type:plasmid
Tissue Supplier:
Library Producer:Michael Brownstein / Ted Usdin Laboratory